green fluorescent protein

1 زیست شناسی:: پروتئین فلوئوروسانس سبز، پروتئین فلورسنت سبز

To test this fusion protein, the green fluorescent protein (GFP) reporter gene was employed as a target gene and was integrated into a CHO chromosome under the control of the CMV promoter with the LexA­binding sequence inserted upstream. Production of GFP, determined by measuring the emission of green fluorescence, was severalfold higher in cells that expressed the LexA-histone acetyltransferase fusion protein than (A) To increase the expression of a recombinant protein, HAT was directed to the site of target gene (GFP gene) insertion in a mammalian chromosome. pression of GFP. SV40 promoter (PSV40) increased expression of GFP 6­fold compared to production of the LexA protein alone.،Kragl and colleagues (2009) performed an experiment in which they transplanted limb tissue from a salamander whose cells expressed green fluorescent protein (GFP) into different regions of limbs of normal salamanders that did not have the GFP transgene (FIGURE 22.19). If they transplanted the GFP-expressing limb cartilage into a salamander limb that did not contain the GFP transgene, the GFP-expressing cartilage would integrate normally into the limb skeleton.

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